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Sample submission

All samples submitted to the CCP Core Facility must be accompanied with a sample submission form.

 

  • We require a valid purchase order to be attached to the submission form.
  • Please use this submission form for MS/MS.
  • Please use this submission form for MALDI MS.
  • Please use this submission form for MALDI Mass Fingerprint/Protein Identification from SDS gels 
  • If you are from the Biochemistry Department, please use this submission form (grant code required).

 

For additional information please contact Dr Mike Deery.

 


 

Pricing guidance notes:

  1. Purified single proteins from gels or solution generally only require a 60 minute LC-MS/MS run.  Similarly, 2D gels spots will only require the 60 minute run.
  2. More complex samples (e.g complex pull-downs or total lysates which have been run on a gel or pull-downs containing proteins in solution) generally require a longer 120 min to reduce the complexity of the sample and generate a greater number of protein identifications.

Analysis prices:

Gel-based methods

Academic (£)Industry (£)

Running gels*

1D minigel 55 110
2D minigel 110 220
2D (13cm) 140 280
2D (24cm) 175 350
DIGE** 350 700
Image analysis (DeCyder) 55 per hour 110 per hour

* Including sample preparation, gel preparation, staining with coomassie or silver and scanning

** Including sample preparation, labelling, 1st and 2nd dimention and scanning

 

LC-MS/MS

Sequencing of proteins*Academic (£)Industry (£)

< 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 150 225
120+ min LC run 175 260

> 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 120 180
120+ min LC run 150 210

Sequencing of proteins from solutions**

60 min LC-MS/MS run 150 225
120+ min LC run 175 260

* Includes sample preparation and database searching

** Solutions must be free of salts and detergents

 

TMT quantitation

Sequencing of proteinsAcademic (£)Industry (£)
Sample preparation 6plex* 750 975
Sample preparation 10plex* 950 1175
LC-MS/MS run** 185 310
Quantitation analysis 50 per hour 70 per hour

* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 30 fractions

** Long gradient, including Mascot searching

 

MALDI-TOF 

Prices for MALDI Intact Mass analysis of solution samples.

This includes sample preparation/clean-up, analysis by mass spectrometry and interpretation of results. Prices are per sample.

  • Biochemistry Department - £30
  • Cambridge University Departments - £48
  • External Academic Clients - £60+VAT
  • Industry (where capacity permits) - £180+VAT 

 

MALDI Fingerprinting prices 

This includes sample preparation, digestion, sample clean-up, analysis by mass spectrometry and searching of the data against databases. Prices are per sample (gel band). Where possible, a few selected peaks will be subjected to MS/MS fragmentation to enhance the database match. 

  • Biochemistry Department - £48
  • Cambridge University Departments - £72
  • External Academic Clients - £100+VAT
  • Industry (where capacity permits) - £145+VAT