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Sample submission

All samples submitted to the CCP Core Facility must be accompanied with a sample submission form and Purchase Order (or grant code for Biochemistry only).

 

  • We require a valid purchase order to be attached to the submission form.
  • Please use this submission form for MS/MS.
  • Please use this submission form for MALDI Intact Protein Analysis.
  • Please use this submission form for MALDI Mass Fingerprint/Protein Identification from SDS gels.
  • If you are from the Biochemistry Department, please use this submission form for MS/MS (grant code required).

 

For additional information please contact Dr Mike Deery.

 


 

Pricing guidance notes:

  1. Purified single proteins from gels or solution generally only require a 60 minute LC-MS/MS run.  Similarly, 2D gels spots will only require the 60 minute run.
  2. More complex samples (e.g complex pull-downs or total lysates which have been run on a gel or pull-downs containing proteins in solution) generally require a longer 120 min to reduce the complexity of the sample and generate a greater number of protein identifications.
  3. The cost of digestion with trypsin is included in the prices below.  Use of any other enzyme(s) will result in an additional charge.  Please ask for a quotation before filling in the appropriate submission form.
  4. The prices shown below are for each LC-MS/MS run - please consider this when calculating the total cost of the experiment.

Analysis prices:

Gel-based methods

Academic (£)Industry (£)

Running gels*

1D minigel 55 110
2D minigel 110 220
2D (13cm) 140 280
2D (24cm) 175 350
DIGE** 350 700
Image analysis (DeCyder) 55 per hour 110 per hour

* Including sample preparation, gel preparation, staining with coomassie or silver and scanning

** Including sample preparation, labelling, 1st and 2nd dimention and scanning

 

LC-MS/MS

Sequencing of proteins*Academic (£)Industry (£)

< 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 150 225
120+ min LC run 175 260

> 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 120 180
120+ min LC run 150 210

Sequencing of proteins from solutions**

60 min LC-MS/MS run 150 225
120+ min LC run 175 260

* Includes sample preparation and database searching

** Solutions must be free of salts and detergents

 

TMT quantitation

Sequencing of proteinsAcademic (£)Industry (£)
Sample preparation 6plex* 750 975
Sample preparation 10plex* 950 1175
LC-MS/MS run** 185 310
Quantitation analysis 50 per hour 70 per hour

* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 30 fractions

** Long gradient, including Mascot searching - price per fraction

 

MALDI-TOF 

Prices for MALDI Intact Mass analysis of solution samples.

This includes sample preparation/clean-up, analysis by mass spectrometry and interpretation of results. Prices are per sample.

  • Biochemistry Department - £30
  • Cambridge University Departments - £48
  • External Academic Clients - £60+VAT
  • Industry (where capacity permits) - £180+VAT 

 

MALDI Fingerprinting prices 

This includes sample preparation, digestion, sample clean-up, analysis by mass spectrometry and searching of the data against databases. Prices are per sample (gel band). Where possible, a few selected peaks will be subjected to MS/MS fragmentation to enhance the database match. 

  • Biochemistry Department - £48
  • Cambridge University Departments - £72
  • External Academic Clients - £100+VAT
  • Industry (where capacity permits) - £145+VAT