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Sample submission

All samples submitted to the CCP Core Facility must be accompanied with a sample submission form and Purchase Order (or grant code for Biochemistry only).
  • We require a valid purchase order (made out to University of Cambridge / Department of Biochemistry) to be attached to the submission form. 
  • Please use this submission form for LC-MS/MS.
  • Please use this submission form for MALDI Intact Protein Analysis.
  • If you are from the Biochemistry Department, please use this submission form for LC-MS/MS (grant code required).

Please note:  there will be no MALDI service between 15th - 17th May 2019. 

Samples should be delivered to the Core Facility address below.

For general enquiries or questions please contact

The CCP core now offers a 'Quantitative Phosphoproteomics' service. Please contact 

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Pricing guidance notes:

  1. Purified single proteins from gels or solution generally only require a 60 minute LC-MS/MS run. Similarly, 2D gels spots will only require the 60 minute run.
  2. More complex samples (e.g complex pull-downs or total lysates which have been run on a gel or pull-downs containing proteins in solution) generally require a longer 120 min to reduce the complexity of the sample and generate a greater number of protein identifications.
  3. The cost of digestion with trypsin is included in the prices below. Use of any other enzyme(s) will result in an additional charge. Please ask for a quotation before filling in the appropriate submission form.
  4. The prices shown below are for each LC-MS/MS run - please consider this when calculating the total cost of the experiment.
  5. VAT will be payable on all external orders as exemption does not cover supply of 'information' (i.e. results). No VAT is payable by University of Cambridge customers.

 

Analysis prices:

Gel-based methods

Academic (£)Industry (£)

Running gels*

1D minigel 65 120
2D minigel 120 230
2D (13cm) 150 290
2D (24cm) 185 360
Protein quantitation (BCA assay)
                         60 110

* Including sample preparation, gel preparation, staining with coomassie or silver and scanning

 

 

LC-MS/MS

Sequencing of proteins*Academic (£)Industry (£)

< 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 160 235
120+ min LC run 185 270

> 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 130 190
120+ min LC run 160 220

Sequencing of proteins from solutions**

60 min LC-MS/MS run 160 235
120+ min LC run 185 270

* Includes sample preparation and database searching

** Solutions must be free of salts and detergents

 

TMT quantitation

A complex mixture of proteins in solution can be analysed for each sample. Buffer composition and protein concentration should be supplied for each sample (ensuring the correct kit is used for the buffer present).

Sequencing of proteinsAcademic (£)Industry (£)
Sample preparation 6plex* 850 1075
Sample preparation 10plex* 1050 1275
LC-MS/MS run** 195 320
Quantitation analysis 60 per hour 80 per hour

* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 40 fractions

** Long gradient, including Mascot searching - price per fraction

 

Quantitative Phosphoproteomics

The enrichment of the phosphoproteome will result in 5 fractions: 1 multiphosphorylated peptide fraction and 4 Monophosphorylated peptide fractions. The multiphosphorylated peptide fraction requires a 4 hour LC-MS/MS run

Sequencing of proteinsAcademic (£)Industry (£)
Sample preparation 6plex* 850 1075
Sample preparation 10plex* 1050 1275
Phosphoproteome Enrichment 1100 1700
LC-MS/MS 2-hour run** 195 320
LC-MS/MS 4-hour run** 300 425
Quantitation analysis 60 per hour 80 per hour

* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 40 fractions

** Long gradient, including Mascot searching - price per fraction

 

MALDI-TOF 

Prices for MALDI Intact Mass analysis of solution samples. 

Provide ≥30μl of >10μM sample; higher concns. give better results and avoid adding detergents.

Price includes sample preparation/clean-up, analysis by mass spectrometry and interpretation of results.

Prices are per sample.

  • Biochemistry Department - £30
  • Cambridge University Departments - £48
  • External Academic Clients - £60+VAT
  • Industry (where capacity permits) - £180+VAT 

 

MALDI Fingerprinting prices 

This includes sample preparation, digestion, sample clean-up, analysis by mass spectrometry and searching of the data against databases. Where possible, a few selected peaks will be subjected to MS/MS fragmentation to enhance the database match. 

Prices are per sample (gel band).

  • Biochemistry Department - £48
  • Cambridge University Departments - £72
  • External Academic Clients - £100+VAT
  • Industry (where capacity permits) - £145+VAT 

 

Core Facility Address :

  • Cambridge Centre for Proteomics
    Cambridge System Biology Centre
    University of Cambridge
    Tennis Court Road
    Cambridge CB2 1QR