Gel-based methods

Academic (£) Industry (£)
Running gels*
1D minigel 55 110
2D minigel 110 220
2D (13cm) 140 280
2D (24cm) 175 350
DIGE** 350 700
Image analysis (DeCyder) 55 per hour 110 per hour

* Including sample preparation, gel preparation, staining with coomassie or silver and scanning

** Including sample preparation, labelling, 1st and 2nd dimention and scanning

LC-MS/MS

Sequencing of proteins* Academic (£) Industry (£)
< 5 bands/spots (price per band/spot)
60 min LC-MS/MS run 150 225
120+ min LC run 175 260
> 5 bands/spots (price per band/spot)
60 min LC-MS/MS run 120 180
120+ min LC run 150 210
Sequencing of proteins from solutions**
60 min LC-MS/MS run 150 225
120+ min LC run 175 260

* Includes sample preparation and database searching

** Solutions must be free of salts and detergents

TMT quantitation

Sequencing of proteins Academic (£) Industry (£)
Sample preparation 6plex* 750 975
Sample preparation 10plex* 950 1175
LC-MS/MS run** 185 310
Quantitation analysis 50 per hour 70 per hour

* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 30 fractions

** Long gradient, including Mascot searching