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Proteomics Core Facility

The facility offers considerable expertise to researchers in industrial and academic environments in gel and mass spectrometry-based proteomics.  A list of recent publications arising from collaborations with the CCP can be found here.
Further information about the facility can be found in the brochure, which includes details on the types of experiments which can be performed at the facility as well as available instrumentation and price list.

 

LC-MS/MS

Sequencing of proteins*Academic (£)Industry (£)

< 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 160 235
120+ min LC run 185 270

> 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 130 190
120+ min LC run 160 220

Sequencing of proteins from solutions**

60 min LC-MS/MS run 160 235
120+ min LC run 185 270

* Includes sample preparation and database searching

** Solutions must be free of salts and detergents

 

TMT quantitation

A complex mixture of proteins in solution can be analysed for each sample. Buffer composition and protein concentration should be supplied for each sample (ensuring the correct kit is used for the buffer present).

Sequencing of proteinsAcademic (£)Industry (£)
Sample preparation 6plex* 850 1075
Sample preparation 10plex* 1050 1275
LC-MS/MS run** 195 320
Quantitation analysis 60 per hour 80 per hour

* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 40 fractions

** Long gradient, including Mascot searching - price per fraction

 

Quantitative Phosphoproteomics

The enrichment of the phosphoproteome will result in 5 fractions: 1 multiphosphorylated peptide fraction and 4 Monophosphorylated peptide fractions. The multiphosphorylated peptide fraction requires a 4 hour LC-MS/MS run

Sequencing of proteinsAcademic (£)Industry (£)
Sample preparation 6plex* 850 1075
Sample preparation 10plex* 1050 1275
Phosphoproteome Enrichment 1100 1700
LC-MS/MS 2-hour run** 195 320
LC-MS/MS 4-hour run** 300 425
Quantitation analysis 60 per hour 80 per hour

* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 40 fractions

** Long gradient, including Mascot searching - price per fraction

 

Gel-based methods running gels at CCP

Academic (£)Industry (£)

Running gels*

1D minigel 65 120
2D minigel 120 230
2D (13cm) 150 290
Protein quantitation (BCA assay)
60 110

* Including sample preparation, gel preparation, staining with coomassie or silver and scanning

 

Pricing guidance notes:

  1. 2D gels spots or purified single proteins, from gels or solution, generally only require a 60 minute LC-MS/MS run.
  2. More complex samples (e.g complex pull-downs or total lysates which have been run on a gel or pull-downs containing proteins in solution) generally require a longer 120 min to reduce the complexity of the sample and generate a greater number of protein identifications.
  3. The cost of digestion with trypsin is included in the prices below. Use of any other enzyme(s) will result in an additional charge. Please ask for a quotation before filling in the appropriate submission form.
  4. The prices shown below are for each LC-MS/MS run, excluding VAT, - please consider this when calculating the total cost of the experiment.
  5. VAT will be payable on all external orders as exemption does not cover supply of 'information' (i.e. results). No VAT is payable by University of Cambridge customers.

 

Contact us

 

- Facility Manager

Tel: 01223 (7)60253

- Phosphoproteomics

Tel: 01223 (7)60253

Services Offered

We require a valid purchase order (made out to University of Cambridge / Department of Biochemistry) to be attached to the submission form.

  • LC-MS/MS  submission form
  • Biochemistry Department, LC-MS/MS (grant code required) submission form 
  • TMT Quantitation - Contact Dr Mike Deery
  • Quantitative Phosphoproteomics - Contact Dr Anja Andrejeva

 

Address

Cambridge Centre for Proteomics
Milner Therapeutics Institute
Jeffrey Cheah Biomedical Centre 
University of Cambridge
Puddicombe Way
Cambridge
CB2 0AW