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Cambridge Centre for Proteomics

 
Source

 The Core Facility have now relocated to:

Cambridge Centre for Proteomics, c/o MRC-Toxicology Unit, Tennis Court Road, Cambridge CB2 1QR

Samples can be delivered in person to the box in reception by prior arrangement only due to reduced staffing levels because of Covid 19 restrictions.

 NB samples delivered in person must be sealed inside a clean plastic bag or they will not be accepted    

The facility offers considerable expertise to researchers in industrial and academic environments in gel and mass spectrometry-based proteomics.  A list of recent publications arising from collaborations with the CCP can be found here.

Further information about the facility can be found in the brochure, which includes details on the types of experiments which can be performed at the facility as well as available instrumentation and price list.

Our new prices and information for 2022 can be found in the 2022 brochure.

 

LC-MS/MS

Sequencing of proteins* Academic (£) Industry (£)

< 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 160 235
120+ min LC run 185 270

> 5 bands/spots (price per band/spot)

60 min LC-MS/MS run 130 190
120+ min LC run 160 220

Sequencing of proteins from solutions**

60 min LC-MS/MS run 160 235
120+ min LC run 185 270

* Includes sample preparation and database searching

** Solutions must be free of salts and detergents

 

TMT quantitation

A complex mixture of proteins in solution can be analysed for each sample. Buffer composition and protein concentration should be supplied for each sample (ensuring the correct kit is used for the buffer present).

Sequencing of proteins Academic (£) Industry (£)
Sample preparation 6plex* 850 1075
Sample preparation 10plex* 1050 1275
Sample preparation 11plex* 1200 1455
Sample preparation 16plex* 1600 1875
LC-MS/MS 2-hour run** 195 320
Quantitation analysis 60 per hour 80 per hour

* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 40 fractions. 

** Long gradient, including Mascot searching - price per fraction

 

Gel-based methods running gels at CCP

  Academic (£) Industry (£)

Running gels*

1D minigel 65 120
2D minigel 120 230
2D (13cm) 150 290
Protein quantitation (BCA assay)    
  60 110

* Including sample preparation, gel preparation, staining with coomassie or silver and scanning

 

Pricing guidance notes:

  1. 2D gels spots or purified single proteins, from gels or solution, generally only require a 60 minute LC-MS/MS run.
  2. More complex samples (e.g complex pull-downs or total lysates which have been run on a gel or pull-downs containing proteins in solution) generally require a longer 120 min to reduce the complexity of the sample and generate a greater number of protein identifications.
  3. The cost of digestion with trypsin is included in the prices below. Use of any other enzyme(s) will result in an additional charge. Please ask for a quotation before filling in the appropriate submission form.
  4. The prices shown above are for each LC-MS/MS run, excluding VAT, - please consider this when calculating the total cost of the experiment.
  5. VAT will be payable on all external orders as exemption does not cover supply of 'information' (i.e. results). No VAT is payable by University of Cambridge customers.

 

Contact us

- Facility Manager

Tel: 01223 (7)60253

Services Offered

We require a valid purchase order (made out to University of Cambridge / Department of Biochemistry) to be attached to the submission form.

 

Address

Cambridge Centre for Proteomics
c/o MRC-Toxicology Unit
Tennis Court Road
Cambridge
CB2 1QR