LC-MS/MS
| Sequencing of proteins* | Academic (£) | Industry (£) |
|---|---|---|
|
< 5 bands/spots (price per band/spot) |
||
| 60 min LC-MS/MS run | 160 | 235 |
| 120+ min LC run | 185 | 270 |
|
> 5 bands/spots (price per band/spot) |
||
| 60 min LC-MS/MS run | 130 | 190 |
| 120+ min LC run | 160 | 220 |
|
Sequencing of proteins from solutions** |
||
| 60 min LC-MS/MS run | 160 | 235 |
| 120+ min LC run | 185 | 270 |
* Includes sample preparation and database searching
** Solutions must be free of salts and detergents
TMT quantitation
A complex mixture of proteins in solution can be analysed for each sample. Buffer composition and protein concentration should be supplied for each sample (ensuring the correct kit is used for the buffer present).
| Sequencing of proteins | Academic (£) | Industry (£) |
|---|---|---|
| Sample preparation 6plex* | 850 | 1075 |
| Sample preparation 10plex* | 1050 | 1275 |
| Sample preparation 11plex* | 1200 | 1455 |
| Sample preparation 16plex* | 1600 | 1875 |
| LC-MS/MS run** | 195 | 320 |
| Quantitation analysis | 60 per hour | 80 per hour |
* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 40 fractions.
** Long gradient, including Mascot searching - price per fraction
Quantitative Phosphoproteomics
The enrichment of the phosphoproteome will result in 5 fractions: 1 multiphosphorylated peptide fraction and 4 Monophosphorylated peptide fractions. The multiphosphorylated peptide fraction requires a 4 hour LC-MS/MS run
| Sequencing of proteins | Academic (£) | Industry (£) |
|---|---|---|
| Sample preparation 6plex* | 850 | 1075 |
| Sample preparation 10plex* | 1050 | 1275 |
| Sample preparation 11plex* | 1200 | 1455 |
| Sample preparation 16plex* | 1600 | 1875 |
| Phosphoproteome Enrichment | 1100 | 1700 |
| LC-MS/MS 2-hour run** | 195 | 320 |
| LC-MS/MS 4-hour run** | 300 | 425 |
| Quantitation analysis | 60 per hour | 80 per hour |
* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 40 fractions
** Long gradient, including Mascot searching - price per fraction
Gel-based methods running gels at CCP
| Academic (£) | Industry (£) | |
|---|---|---|
|
Running gels* |
||
| 1D minigel | 65 | 120 |
| 2D minigel | 120 | 230 |
| 2D (13cm) | 150 | 290 |
| Protein quantitation (BCA assay) | ||
| 60 | 110 | |
* Including sample preparation, gel preparation, staining with coomassie or silver and scanning
Pricing guidance notes:
- 2D gels spots or purified single proteins, from gels or solution, generally only require a 60 minute LC-MS/MS run.
- More complex samples (e.g complex pull-downs or total lysates which have been run on a gel or pull-downs containing proteins in solution) generally require a longer 120 min to reduce the complexity of the sample and generate a greater number of protein identifications.
- The cost of digestion with trypsin is included in the prices below. Use of any other enzyme(s) will result in an additional charge. Please ask for a quotation before filling in the appropriate submission form.
- The prices shown above are for each LC-MS/MS run, excluding VAT, - please consider this when calculating the total cost of the experiment.
- VAT will be payable on all external orders as exemption does not cover supply of 'information' (i.e. results). No VAT is payable by University of Cambridge customers.