The Core Facility have now relocated to:
Cambridge Centre for Proteomics, c/o MRC-Toxicology Unit, Tennis Court Road, Cambridge CB2 1QR
Samples can be delivered in person to the box in reception by prior arrangement only due to reduced staffing levels because of Covid 19 restrictions.
NB samples delivered in person must be sealed inside a clean plastic bag or they will not be accepted
The facility offers considerable expertise to researchers in industrial and academic environments in gel and mass spectrometry-based proteomics. A list of recent publications arising from collaborations with the CCP can be found here.
Further information about the facility can be found in the brochure, which includes details on the types of experiments which can be performed at the facility as well as available instrumentation and price list.
Our new prices and information for 2022 can be found in the 2022 brochure.
LC-MS/MS
| Sequencing of proteins* | Academic (£) | Industry (£) |
|---|---|---|
|
< 5 bands/spots (price per band/spot) |
||
| 60 min LC-MS/MS run | 160 | 235 |
| 120+ min LC run | 185 | 270 |
|
> 5 bands/spots (price per band/spot) |
||
| 60 min LC-MS/MS run | 130 | 190 |
| 120+ min LC run | 160 | 220 |
|
Sequencing of proteins from solutions** |
||
| 60 min LC-MS/MS run | 160 | 235 |
| 120+ min LC run | 185 | 270 |
* Includes sample preparation and database searching
** Solutions must be free of salts and detergents
TMT quantitation
A complex mixture of proteins in solution can be analysed for each sample. Buffer composition and protein concentration should be supplied for each sample (ensuring the correct kit is used for the buffer present).
| Sequencing of proteins | Academic (£) | Industry (£) |
|---|---|---|
| Sample preparation 6plex* | 850 | 1075 |
| Sample preparation 10plex* | 1050 | 1275 |
| Sample preparation 11plex* | 1200 | 1455 |
| Sample preparation 16plex* | 1600 | 1875 |
| LC-MS/MS 2-hour run** | 195 | 320 |
| Quantitation analysis | 60 per hour | 80 per hour |
* Includes protein precipitation, reduction/alkylation/digestion, TMT Labelling, 1st dimensionLC and collection of up to 40 fractions.
** Long gradient, including Mascot searching - price per fraction
Gel-based methods running gels at CCP
| Academic (£) | Industry (£) | |
|---|---|---|
|
Running gels* |
||
| 1D minigel | 65 | 120 |
| 2D minigel | 120 | 230 |
| 2D (13cm) | 150 | 290 |
| Protein quantitation (BCA assay) | ||
| 60 | 110 | |
* Including sample preparation, gel preparation, staining with coomassie or silver and scanning
Pricing guidance notes:
- 2D gels spots or purified single proteins, from gels or solution, generally only require a 60 minute LC-MS/MS run.
- More complex samples (e.g complex pull-downs or total lysates which have been run on a gel or pull-downs containing proteins in solution) generally require a longer 120 min to reduce the complexity of the sample and generate a greater number of protein identifications.
- The cost of digestion with trypsin is included in the prices below. Use of any other enzyme(s) will result in an additional charge. Please ask for a quotation before filling in the appropriate submission form.
- The prices shown above are for each LC-MS/MS run, excluding VAT, - please consider this when calculating the total cost of the experiment.
- VAT will be payable on all external orders as exemption does not cover supply of 'information' (i.e. results). No VAT is payable by University of Cambridge customers.